Figure 4 | Scientific Reports

Figure 4

From: The tumor suppressor miR-642a-5p targets Wilms Tumor 1 gene and cell-cycle progression in prostate cancer

Figure 4

WT1 is a direct target of miR-642a-5p in PCa cells. (a) The 3′UTR of WT1 has three putative miR-642a-5p seed sites as predicted by TargetScan 7.2. (b) Schematic of the 3′UTR of WT1 (not to scale). Depiction of the GeneCopoeia target clone, which contains only the first 1293 base pairs of the 3′UTR, is with green shading. The grey shaded boxes indicate the miR-642a-5p seed sites. (c) Luciferase reporter gene analysis of the 3′UTR of the putative miR-642a-5p target WT1 in 22Rv1 and LNCaP PCa cells transiently overexpressing miR-642a-5p or miR-NC (20 nM). DOHH and miR-642a-5p perfect targets are positive controls. Error bars = SD; n = 3; **p < 0.005.

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