Figure 6
WT1 overexpression increases colony formation and miR-642a-5p rescues this effect. (a) RT-qPCR analysis of WT1 gene expression following stable or transient LeGO-iT2-WT1-203 or LeGO-iT2-Empty plasmids, and overexpression of miR-642a-5p or miR-NC in 22Rv1 and LNCaP PCa cells. Expression of WT1 is normalized to HPRT housekeeping gene expression, calculated using the 2−ΔΔCt method, and relative to Empty vector + miR-NC. Error bars = SE; n = 3; *p < 0.05, **p < 0.005 relative to Empty vector + miR-NC. #p < 0.05 WT1-203 + miR-NC relative to WT1-203 + miR-642a-5p. (b) Colony formation assay of 22Rv1 PCa cells 14 days post transient WT1 overexpression/empty vector transfection and miR-NC/miR-642a-5p (30 nM) co-transfection. Error bars = SD; n = 3; **p < 0.005 relative to Empty vector + miR-NC. ##p < 0.005 WT1-203 + miR-NC relative to WT1-203 + miR-642a-5p.
