Figure 2
From: Functionally diverse heteromeric traps for ligands of the transforming growth factor-β superfamily
Profiles of ligand dissociation kinetics for heterodimeric ligand traps vary with type I receptor component. (A) Domain schematics depicting variants based on naturally occurring pairings of TGF-β superfamily type I receptor ECDs with ActRIIB ECD. The Fc domains of heterodimeric fusion proteins are modified (Mod) to promote desired pairing of monomers during cellular production. H6, histidine hexamer. (B–D) Ligand sequestration profiles for ActRIIB-Fc-based heterodimeric traps vary markedly with the identity of the partnered type I receptor ECD. Semi-schematic graphs depict the change in off-rate (kd) of key ligands, as determined by surface plasmon resonance (see Table 1 for exact values), when one ECD in the IIB-Fc:IIB-Fc homodimer is replaced with ALK4 (B), ALK7 (C), or ALK3 (D).
