Table 2 Mean IC50 values for ligand inhibition by Fc-fusion constructs in a reporter-gene assay.

From: Functionally diverse heteromeric traps for ligands of the transforming growth factor-β superfamily

Ligand

Reference

Heterodimers

Homodimers

IIB-Fc:IIB-Fc

IIB-Fc:ALK4-Fc

IIB-Fc:ALK7-Fc

IIB-Fc:ALK3-Fc

ALK4-Fc:ALK4-Fc

ALK7-Fc:ALK7-Fc

ALK3-Fc:ALK3-Fc

Activin A

0.11 ± 0.01

0.19 ± 0.04

2.86 ± 0.60

2.62 ± 0.15

 > 100

ND

ND

Activin B

0.06 ± 0.01

0.16 ± 0.01

0.26 ± 0.05

1.65 ± 0.02

ND

ND

ND

GDF8

0.76 ± 0.10

2.04 ± 0.04

9.15 ± 1.17

10.78 ± 0.31

ND

ND

ND

GDF11

0.09 ± 0.01

0.34 ± 0.02

2.34 ± 0.67

2.42 ± 0.07

ND

ND

ND

BMP9

0.57 ± 0.06

8.47 ± 1.09

31.02 ± 4.25

23.40 ± 3.34

ND

ND

ND

BMP10

0.03 ± 0.00

0.70 ± 0.19

2.01 ± 0.29

1.10 ± 0.14

ND

ND

ND

  1. Values are means ± SEMs (n = 3), expressed in nM. ND, no inhibition determined at the highest experimental concentration for each molecule tested.
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