Table 3 Relative expression (Log2FC) and statistical significance between normal and FHS-affected broilers obtained with qPCR and comparison with RNA-Seq results for 11 candidate genes.

From: Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens

Gene

qPCR

p-value

RNA-Seq

FDR

AvBD1

3.21

0.005

3.55

1.23E-07

AvBD2

3.65

0.006

3.32

0.0006

FBN2

0.52

0.227

− 1.08

0.037

ANK1

2.45

0.014

2.12

0.023

RHAG

3.04

0.006

2.44

0.010

CSF3R

0.86

0.356

2.28

0.003

EPX

3.48

0.007

4.27

3.23E-09

ADA

2.14

0.003

1.72

0.008

COL13A1

− 0.22

0.562

− 1.30

0.038

S100A9

2.51

0.093

4.04

1.84E-06

PSPH

− 1.13

0.218

− 1.43

0.012

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