Table 4 Primers used for the qPCR analysis of the target candidate genes for FHS in the femur articular cartilage of broilers.
Gene | Ensembl ID | Primer sequences (5'-3') |
|---|---|---|
AvBD1 | ENSGALG00000022815 | F: CAGGATCCTCCCAGGCTCTA |
avian beta-defensin 1 | R: GATGAGAGTGAGGGAAGGGC | |
AvBD2 | ENSGALG00000016669 | F: TTCTCCAGGGTTGTCTTCGC |
avian beta-defensin 2 | R: TGCATTCCAAGGCCATTTGC | |
FBN2 | ENSGALG00000014686 | F: TGCATCGATAGCCTGAAGGG |
fibrillin 2 | R: CTAATTCACACCGCTCACATGG | |
ANK1 | ENSG00000029534 | F: CCACCATCCCACCATTCAGT |
Ankyrin 1 | R: ACGGTCACAAACTCCAGCAT | |
PSPH | ENSGALG00000002397 | F:CAGGAATACGGGAGCTGGTG |
phosphoserine phosphatase | R: CCCAGAGACCAGGAAGACCT | |
EPX | ENSGALG00000043254 | F:AAAGGAGGTGGCATTGACCC |
eosinophil peroxidase | R: GCCACGCTGCATGTTAAGAG | |
ADA | ENSGALG00000004170 | F:TTCGGCAAGAAAAGAGGGGT |
adenosine deaminase | R: GTGTTTGGTAGCTGACGTGC | |
COL13A1 | ENSGALG00000004286 | F:CCAAGCAAGGACTAGACACTCA |
collagen type XIII alpha 1 chain | R: ACCCTTCATGCCATGTCTTCC | |
CSF3R | ENSGALG00000002112 | F: TCATCCGGGACAGCATTGAG |
colony stimulating factor 3 receptor | R: TGTAGAGGGGGTACACCGAG | |
RHAG | ENSGALG00000016684 | F:TCTGGAGATCACGGCCTTTG |
Rh associated glycoprotein | R:GCTCCAATATCTGTGGCCTGA | |
S100A9 | ENSGALG00000024272 | F:GGGGACAAAGACACCCTGAC |
S100 calcium binding protein A9 | R:TTCACGTGCTTCAGGTAGTTGG |
