Table 4 Effect of variables on L-asparaginase production by Penicillium cerradense evaluated by Fractional Factorial Design.
Variable | Effect | Standard error | t calc | p value |
|---|---|---|---|---|
Mean | 1.65 | 0.08 | 21.71 | 0.00 |
Curvature | − 1.29 | 0.34 | − 3.79 | 0.00 |
Model | – | – | − 1.43 | 0.19 |
L-proline (X1) | 0.01 | 0.15 | 0.03 | 0.97 |
Peptone (X2) | − 0.09 | 0.15 | − 0.58 | 0.58 |
Sucrose (X3) | − 0.25 | 0.15 | − 1.64 | 0.13 |
Urea (X4) | − 0.06 | 0.15 | − 0.39 | 0.71 |
Ammonium sulfate (X5) | 0.43 | 0.15 | 2.84 | 0.02 |
Yeast extract (X6) | − 0.22 | 0.15 | − 1.48 | 0.17 |
Glucose (X7) | − 0.13 | 0.15 | − 0.89 | 0.40 |
L-asparagine (X8) | 0.08 | 0.15 | 0.53 | 0.60 |
Lack of Fit | – | – | 2.58 | 0.04 |
