Table 5 Levels of the independent variables of the PBD experimental design to identify the influential factors on L-asparaginase production.
Variables | Symbol | Levels | ||
|---|---|---|---|---|
− 1 (%) | 0 (%) | + 1 (%) | ||
L-asparagine | X1 | 1.0 | 2.0 | 3.0 |
L-proline | X2 | 1.0 | 2.0 | 3.0 |
Urea | X3 | 0.1 | 0.3 | 0.5 |
Sodium nitrate | X4 | 1.0 | 1.75 | 2.5 |
Yeast extract | X5 | 0.1 | 0.3 | 0.5 |
Ammonium sulfate | X6 | 0.5 | 1.0 | 1.5 |
Peptone | X7 | 1.0 | 1.5 | 2.0 |
Glucose | X8 | 0.0 | 0.1 | 0.2 |
Sucrose | X9 | 0.0 | 0.1 | 0.2 |
Malt extract | X10 | 0.5 | 1.25 | 2.0 |
Potassium chloride | X11 | 0.01 | 0.0175 | 0.025 |
