Figure 1 | Scientific Reports

Figure 1

From: Regulation of low-density lipoprotein receptor expression in triple negative breast cancer by EGFR-MAPK signaling

Figure 1

EGF stimulates LDL-cholesterol uptake and induces the expression of LDLR in MDA-MB-468 cells. (a) Representative images and quantification of fluorescent LDL-cholesterol uptake by MDA468 cells after stimulation with 10 ng/mL EGF for 5 h, *indicates p = 0.0003 between groups, n = 10 fields of view per group. (b,c) Representative western blots of LDLR, EGFR and MAPK phosphorylation in MDA468 cells stimulated with 10 ng/mL EGF at the indicated time points. Blots have been cropped for conciseness of presentation and uncropped blots are shown in Supplementary Fig. S7. Quantification of LDLR and phospho-p44/42MAPK are from blots shown in Supplementary Fig. S1 for 2 h and 4 h of EGF stimulation. *Indicates p < 0.05, **indicates p < 0.01, ***indicates p < 0.0001, n = 3 per condition for 2 h and n = 6 per condition for 4 h. (d) Gene expression of LDLR in MDA468 cells stimulated with 10 ng/mL EGF for 2 h, *indicates p = 0.003 between groups, n = 3 per condition. Horizontal line indicates mean of the groups, error bars are S.E.M.

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