Figure 3
EGF-induction of LDLR is cell line-dependent. (a,b) Representative western blots of LDLR in MDA231 cells stimulated with 10 ng/mL EGF over 24 h. Two separate gels using the same samples are shown. Uncropped blots are shown in Supplementary Fig. S8. Quantification of LDLR are from blots shown in Supplementary Fig. S3 for 2 h and 4 h of EGF stimulation, n = 3 per condition, “ns” indicates no statistically significant difference between groups. (b) Gene expression of LDLR in MDA468 and MDA231 cells stimulated with 10 ng/mL EGF for 2 h, n = 3 per condition. **indicates p = 0.006 between groups, “ns” indicates no statistically significant difference between groups, as indicated. (c) MDA468 cells were stimulated with 10 ng/mL EGF for 2 h and analyzed by western blotting for protein expression, ***indicates p < 0.0001 between groups for phospho-Akt/total Akt, and **indicates p = 0.0007 between groups for phospho-STAT3/total STAT3, n = 3 per condition. Uncropped blots are shown in Supplementary Fig. S9. Horizontal line indicates mean of the groups, error bars are S.E.M.
