Figure 4
Activation of STAT3 signaling does not affect LDLR expression in MDA-MB-231 cells or in response to EGF in MDA-MB-468 cells. (a,b) MDA468 cells were pre-treated with 5 µM Stattic for 1 h prior to the addition of BSA (Con) or 10 ng/mL EGF for two hours and analyzed by western blotting for protein expression. Horizontal line indicates mean of the groups, error bars are S.E.M, n = 3 per condition. Brackets indicate groups that are statistically different, *indicates p < 0.05, ***p < 0.001, ****p < 0.0001 between groups, “ns” indicates no statistically significant difference detected between groups, as shown. Uncropped blots are shown in Supplementary Fig. S10. (c) MDA231 cells were treated with 20 ng/mL IL-6 for up to 20 h and analyzed by western blotting for protein expression, n = 2–3 per condition. Uncropped blots are shown in Supplementary Fig. S11.
