Figure 5
MAPK signaling is important for LDLR expression in MDA-MB-231 cells (a,b) MDA468 and MDA231 cells were pre-treated with 20 μM UO126 for 1 h prior to the addition of BSA for two hours and analyzed by western blotting for protein expression, n = 3 per condition. Two separate gels using the same samples are shown. Uncropped blots are shown in Supplementary Fig. S12. (c) Quantitation of western blot of PCSK9 in MDA468 and MDA231 cells pre-treated with UO126, n = 3 per condition. Horizontal line indicates mean of the groups, error bars are S.E.M. Brackets indicate groups significantly different, *indicates p < 0.05 between groups, **p < 0.01, ***p < 0.001, ****p < 0.0001.
