Figure 6
EGF induces the expression of LDLR through the MAPK signaling pathway and stabilizes LDLR mRNA in MDA-MB-468 cells. MDA468 cells were pre-treated with 20 μM UO126 for 1 h prior to stimulation with 10 ng/mL EGF for two hours and analyzed by (a,b) western blotting for protein expression, n = 3 per condition. Two separate gels using the same samples are shown. Uncropped blots are shown in Supplementary Fig. S13. (c) mRNA expression of LDLR in MDA468 cells treated with UO126 and EGF, n = 3 per condition. (d) Representative western blot of ZFP36L1 in MDA468 cells pre-treated with UO126 prior to stimulation with 10 ng/mL EGF. Uncropped blots are shown in Supplementary Fig. S14. Horizontal line indicates mean of the groups, error bars are S.E.M. Brackets indicate groups significantly different, *indicates p < 0.05 between groups, **p < 0.01, ***p < 0.001, ****p < 0.0001.
