Figure 7
RSK1/2 regulates LDLR expression in MDA-MB-468 and MDA-MB-231 cells. MDA468 cells were transfected with control siRNA or siRNA targeted to RSK1 for 72 h and stimulated with 10 ng/mL EGF for two hours prior to analysis by (a,b) western blotting for protein expression, n = 3 per condition. Uncropped blots are shown in Supplementary Fig. S15. (c) Representative western blot of MDA231 cells transfected with control siRNA or siRNA targeted to RSK1 for 72 h. Uncropped blots are shown in Supplementary Fig. S16. n = 3 per condition. (d) Representative western blot of MDA231 cells transfected with control siRNA or siRNA targeted to RSK2 for 72 h. Uncropped blots are shown in Supplementary Fig. S17. n = 6 per condition. Horizontal line indicates mean of the groups, error bars are S.E.M. Brackets indicate groups significantly different, *indicates p < 0.05 between groups, **p < 0.01, ***p < 0.001, ****p < 0.0001.
