Figure 3 | Scientific Reports

Figure 3

From: Comparative transcriptional profiling of canine acanthomatous ameloblastoma and homology with human ameloblastoma

Figure 3

Results of qPCR and IHC validation assays. Panel (A) shows the results of qPCR assays (ddCt, X axis) of 11 genes, plotted against the results of RNA-seq (dlog2FPKM, Y axis), where dlog2FPKM is the difference between the log2(FPKM) value for each sample compared to the average log2(FPKM) for healthy gingiva control samples. The measurements with both gene expression quantification platforms were in excellent agreement for all phenotypes including CAA (blue), COSCC (green) and HGIN (orange); the points lying close to the line were best reproduced between the two experiments. Panel (B) corresponds to a × 40 photomicrograph showing cytoplasmic and nuclear ODAM immunoreactivity (black arrows) of proliferating neoplastic epithelium in one of the CAA cases analyzed.

Back to article page