Table 1 Summary of piggyBac based transformation experiments.

From: The β2Tubulin, Rad50-ATPase and enolase cis-regulatory regions mediate male germline expression in Tribolium castaneum

Exp

Donor plasmid

No of embryos injected

Hatched larvae

Number of adult G0

Female/male G0*

DsRED+ (#) G1

1

piggyBac-β2t

1640

152

80

42/38

15

2

piggyBac-Rad50

1750

151

101

58/43

13

3

piggyBac-Eno

1260

113

52

25/37

7

  1. *Crossed the G0 putative transgenic female with w−/− male in single pair cross or in pool of two to four beetles and vice versa. G1 larvae were screened for the DsRED fluorescence in eye.
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