Figure 3
From: The mitochondrial calcium uniporter promotes arrhythmias caused by high-fat diet
Kv1.5 protein levels are reduced in control hearts on HFD; MCU KO cardiomyocytes are protected from ROS and mitochondrial dysfunction induced by saturated fat. (A) Western blot and graph of Kv1.5 from control hearts, *Indicates sig different from con chow by t-test. Graph values normalized to loading control, n = 4 heart lysates per group. (B) Western blot and graph of Kv2.1 from control hearts. (C) Western blot and graph of Kv4.2 from control hearts. (D) Western blot and graph of Kv1.5 from MCU KO hearts. (E) Isolated control and MCU KO cardiomyocytes, ± palmitate, height is DCF fluorescence minus background, mean + SEM, n = triplicate wells, 2000 cells per well from one heart for each genotype. (F) Control and MCU KO cardiomyocytes, mitosox red signal. (G) Control and MCU KO cardiomyocytes, Rhod2AM signal for mitochondrial calcium. Values for each genotype was normalized to the corresponding control condition for that genotype. (H) Control and MCU KO cardiomyocytes, TMRM signal to indicate mitochondrial inner membrane potential. For all graphs shown, the means are significantly different by ANOVA, *Indicates significantly different from control by post-hoc test. PA = palmitate 200 uM 4 h. For (E–H), similar results were obtained from 3 different isolations for each genotype.
