Table 1 Average Ct values obtained for three target regions from a series of 7 tenfold serial dilution of a clinical specimen previously detected SARS-CoV-2 positive (SC005G) using Meril covid-19 detection kit.
Target 1 (RdRp) | Target 2 (E-gene) | Target 3 (S-gene) | |||||||
|---|---|---|---|---|---|---|---|---|---|
Dilutions | Replicates detected | Average CT-values | SD | Replicates detected | Average CT-values | SD | Replicates detected | Average CT-values | SD |
10–1 | 5/5(100%) | 22.87 | 0.553968 | 5/5(100%) | 23.67 | 0.915341 | 5/5(100%) | 24.25 | 0.604276 |
10–2 | 5/5(100%) | 25.77 | 0.663928 | 5/5(100%) | 26.22 | 0.663099 | 5/5(100%) | 27.62 | 0.934532 |
10–3 | 5/5(100%) | 26.62 | 0.703314 | 5/5(100%) | 27.92 | 0.557692 | 5/5(100%) | 28.43 | 0.79117 |
10–4 | 5/5(100%) | 31.42 | 1.406722 | 5/5(100%) | 32.58 | 0.978673 | 5/5(100%) | 33.45 | 0.41142 |
10–5 | 5/5(100%) | 34.28 | 0.407799 | 5/5(100%) | 33.77 | 0.721249 | 5/5(100%) | 34.30 | 0.44238 |
10–6 | 2/5(40%) | 37.42 | 1.286934 | 2/5(40%) | 38.21 | 0.59397 | 3/5(60%) | 39.53 | 0.600083 |
10–7 | 0/5(0%) | – | – | 0/5(0%) | – | – | 0/5(0%) | – | – |
