Figure 5

(A) Intratumoral VEGF and (B) CA9 expression relative to α-tubulin, with representative Western blot bands (CON, CON-TP, OB, OB-TP). Full-length, uncropped images are included within Supplementary Materials. OB diets significantly increased VEGF, and TP led to reduced VEGF, data which corroborates UMI findings of reduced tumor blood perfusion. (C) Periprostatic adipose expression of TNFα was variable and not associated with diet. (D) Intratumoral TNFα was reduced by TP only when incorporated into the CON diet. *Indicates statistical significance (p < 0.05 by mixed-model ANCOVA with body weight at tumor detection as a covariate, n = 5/dietary group).