Figure 1 | Scientific Reports

Figure 1

From: Characterization and engineering of Streptomyces griseofuscus DSM 40191 as a potential host for heterologous expression of biosynthetic gene clusters

Figure 1

Overview of S. griseofuscus strains and mutations. The positions of genomic inverted repeats are highlighted in blue, BGCs in black and transposases in green. The positions of the identified mutations are highlighted in red. The mutations detected in the wild type Illumina dataset can be considered as a technical noise. The position of the CRISPR-cBEST introduced STOP-codon is indicated with a black triangle. The strains p057_0D and p057_20D relate to the long term cultivation experiment, in which CRISPR-cBEST generated strain S. griseofuscus IHEP81_06602 (p057_0D), that contains an introduced STOP-codon in BGC 30, was transferred 20 consecutive times in liquid ISP2 media without selective pressure, thus generating strain p057_20D. The alignment was created with CLC Genomics Workbench 12.0.3 https://digitalinsights.qiagen.com/ and visualised with Adobe Illustrator 23.0.6 https://www.adobe.com/products/illustrator.html.

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