Figure 3 | Scientific Reports

Figure 3

From: Pharmacological inhibition of cryptochrome and REV-ERB promotes DNA repair and cell cycle arrest in cisplatin-treated human cells

Figure 3

KS15 and SR8278 treatment increase XPA and Wee1 expression in U2OS cells. (a) U2OS cells were treated with DMSO vehicle, 20 µM KS15, 10 µM SR8278, or the combination of KS15 and SR8278 (KS + SR) for 24 h. RNA was purified and analyzed for XPA mRNA expression by RT-qPCR (average and SEM, n = 4). (b) Wee1 mRNA was analyzed as in (a). (c) Cell lysates from cells treated as in (a) were analyzed for XPA protein by immunoblotting (average and SEM, n = 4). (d) Wee1 protein expression was analyzed as in (c). Full-length blots are presented in Supplementary Figure S1. The asterisks indicate differences in mRNA protein expression in comparison to DMSO-treated control cells (p < 0.05; one-way ANOVA).

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