Figure 4 | Scientific Reports

Figure 4

From: Pharmacological inhibition of cryptochrome and REV-ERB promotes DNA repair and cell cycle arrest in cisplatin-treated human cells

Figure 4

Treatment of cells with KS15 and SR8278 treatment promotes the removal of cisplatin–DNA adducts from genomic DNA. (a) U2OS cells were treated with DMSO vehicle or 20 µM KS15 + 10 µM SR8278 along with the indicated concentrations of cisplatin. Genomic DNA was purified from the cells 2 and 24 h later and analyzed by DNA immunoblotting with an anti-cisplatin DNA adduct antibody. Blots were then stained with SybrGold to visualize total DNA. (b) Quantitation of relative cisplatin–DNA adduct signal (average and SEM) at 24 h in relative to the 2 h time point (n = 5). The asterisks indicate significant differences in unrepaired cisplatin adducts (t-test, p < 0.05).

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