Figure 2
From: Platinum chloride-based viability RT-qPCR for SARS-CoV-2 detection in complex samples
Assessment of viability markers on inactivated SARS-CoV-2 viral particles suspended in PBS buffer at different concentrations. RT-qPCR assays targeted N1 region. (a) Comparison of PMAxx and platinum chloride (PtCl4) viability RT-qPCRs on low (ca. 103 gc/mL) gamma- and heat inactivated SARS-CoV-2 viral particles. (b) Viability RT-qPCR optimization using increasing concentration of PtCl4 on high concentrations of gamma-inactivated SARS-CoV-2 viral particles (ca. 105 gc/mL). Dashed grey lines represent RT-qPCR theoretical limit of detection. Asterisks indicate significant difference from untreated control: *p < 0.01; **p < 0.001; ***p < 0.0001.
