Table 1 Statistical analysis of the functional parameters ∆pO2 (difference between pulmonary arterial to pulmonary venous oxygen partial pressure in the perfusate), Cstat and Cdyn (static and dynamic lung compliance), PVR (pulmonary vascular resistance), as well as the ratio between wet and dry weight after EVLP run (wet/dry ration, W/D-ratio) and perfusate measurements of lactate levels, activity of lactate dehydrogenase (LDH) and alkaline phosphatase (AP) after inhalation of a 125 µM (SPH 125), 500 µM (SPH 500) and 10% OGP (OGP) suspension in a 4 h EVLP run.
From: Inhaled sphingosine has no adverse side effects in isolated ventilated and perfused pig lungs
Parameters | EVLP time | OGP | SPH 125 µM | SPH 500 µM | p-value | |||
|---|---|---|---|---|---|---|---|---|
n | Mean ± sd | n | Mean ± sd | n | Mean ± sd | |||
Functional | ||||||||
ΔpO2 | 1 h | 3 | 386 ± 17 | 3 | 291 ± 167 | 3 | 320 ± 92 | n.s |
2 h | 3 | 416 ± 92 | 3 | 295 ± 214 | 3 | 298 ± 97 | n.s | |
3 h | 2 | 371 ± 25 | 3 | 285 ± 183 | 3 | 278 ± 75 | n.s | |
4 h | 3 | 386 ± 105 | 3 | 250 ± 135 | 2 | 273 ± 173 | n.s | |
p-value | n.s | n.s | n.s | |||||
PVR | 1 h | 2 | 530 ± 434 | 3 | 601 ± 772 | 3 | 223 ± 93 | n.s |
2 h | 2 | 466 ± 426 | 3 | 493 ± 579 | 3 | 252 ± 166 | n.s | |
3 h | 2 | 651 ± 707 | 3 | 667 ± 576 | 3 | 310 ± 195 | n.s | |
4 h | 2 | 612 ± 747 | 3 | 742 ± 645 | 2 | 431 ± 531 | n.s | |
p-value | n.s | n.s | n.s | |||||
Cdyn | 1 h | 3 | 27 ± 8 | 3 | 17 ± 2 | 3 | 20 ± 9 | n.s |
2 h | 3 | 24 ± 9 | 3 | 16 ± 6 | 3 | 18 ± 8 | n.s | |
3 h | 3 | 17 ± 4 | 3 | 15 ± 8 | 3 | 20 ± 4 | n.s | |
4 h | 3 | 20 ± 17 | 3 | 12 ± 9 | 2 | 14 ± 12 | n.s | |
p-value | n.s | n.s | n.s | |||||
Cstat | 1 h | 3 | 39 ± 22 | 3 | 19 ± 1 | 3 | 22 ± 10 | n.s |
2 h | 3 | 31 ± 20 | 3 | 18 ± 6 | 3 | 21 ± 8 | n.s | |
3 h | 3 | 32 ± 27 | 3 | 17 ± 6 | 3 | 37 ± 24 | n.s | |
4 h | 3 | 32 ± 36 | 3 | 13 ± 9 | 2 | 21 ± 10 | n.s | |
p-value | n.s | n.s | n.s | |||||
W/D Ratio | 3 | 88.41 ± 2.3 | 3 | 86.98 ± 3.2 | 3 | 87.57 ± 2.3 | n.s | |
Perfusate | ||||||||
Lactate | 1 h | 3 | 1.9 ± 0.42 | 3 | 1.2 ± 0.06 | 3 | 1.4 ± 0.45 | n.s |
2 h | 3 | 2.0 ± 0.49 | 3 | 1.9 ± 0.21 | 3 | 2.4 ± 0.35 | n.s | |
3 h | 3 | 2.6 ± 0.72 | 3 | 2.2 ± 0.15 | 3 | 3.0 ± 0.27 | n.s | |
4 h | 2 | 3.1 ± 0.85 | 3 | 2.7 ± 0.15 | 3 | 3.4 ± 0.20 | n.s | |
p-value | n.s | n.s | n.s | |||||
LDH | Start | 3 | 249 ± 151 | 3 | 200 ± 93 | 3 | 192 ± 164 | n.s |
End | 3 | 326 ± 195 | 3 | 296 ± 127 | 3 | 313 ± 158 | n.s | |
AP | Start | 3 | 7 ± 5 | 3 | 1 ± 2 | 3 | 3 ± 3 | n.s |
End | 3 | 21 ± 25 | 3 | 2 ± 3 | 3 | 5 ± 5 | n.s | |
p-value | n.s | n.s | n.s | |||||
