Table 2 Statistical analysis of mass spectrometry after inhalation of a 10% octylglucopyranoside (OGP), a 125 µM or a 500 µM sphingosine (SPH) solution (5 mL). The range of sphingosine (Sph), ceramide 16 (Cer 16), ceramide 18 (Cer 18), ceramide 20 (Cer20), ceramide 22 (Cer 22), ceramide 24 (Cer 24), ceramide 24:1 (Cer 24:1) and ceramide total (Cer total) in pmol/mg protein.
From: Inhaled sphingosine has no adverse side effects in isolated ventilated and perfused pig lungs
OGP | 125 µM SPH | 500 µM SPH | p-values | ||||
|---|---|---|---|---|---|---|---|
n | Mean ± sd | n | Mean ± sd | n | mean ± sd | ||
Sph | |||||||
Main bronchus | 3 | 68 ± 31 | 3 | 180 ± 55 | 3 | 107 ± 39 | n.s |
Bronchus | 3 | 49 ± 14 | 3 | 105 ± 31 | 3 | 71 ± 56 | n.s |
Periphery | 3 | 134 ± 14 | 3 | 276 ± 192 | 3 | 223 ± 27 | n.s |
Cer 16 | |||||||
Main bronchus | 3 | 696 ± 402 | 3 | 718 ± 361 | 3 | 571 ± 130 | n.s |
Bronchus | 3 | 513 ± 349 | 3 | 965 ± 664 | 3 | 447 ± 159 | n.s |
Periphery | 3 | 628 ± 196 | 3 | 744 ± 276 | 3 | 1507 ± 756 | n.s |
Cer 18 | |||||||
Main bronchus | 3 | 115 ± 74 | 3 | 211 ± 188 | 3 | 126 ± 33 | n.s |
Bronchus | 3 | 132 ± 64 | 3 | 295 ± 75 | 3 | 114 ± 25 | n.s |
Periphery | 3 | 150 ± 70 | 3 | 188 ± 123 | 3 | 418 ± 216 | n.s |
Cer 20 | |||||||
Main bronchus | 3 | 98 ± 79 | 3 | 106 ± 52 | 3 | 67 ± 11 | n.s |
Bronchus | 3 | 75 ± 40 | 3 | 121 ± 64 | 3 | 285 ± 199 | n.s |
Periphery | 3 | 90 ± 41 | 3 | 94 ± 16 | 3 | 127 ± 100 | n.s |
Cer 22 | |||||||
Main bronchus | 3 | 199 ± 140 | 3 | 274 ± 141 | 3 | 195 ± 26 | n.s |
Bronchus | 3 | 129 ± 59 | 3 | 432 ± 191 | 3 | 141 ± 17 | n.s |
Periphery | 3 | 229 ± 79 | 3 | 320 ± 90 | 3 | 555 ± 333 | n.s |
Cer 24 | |||||||
Main bronchus | 3 | 392 ± 271 | 3 | 543 ± 146 | 3 | 453 ± 165 | n.s |
Bronchus | 3 | 209 ± 84 | 3 | 739 ± 354 | 3 | 284 ± 111 | n.s |
Periphery | 3 | 508 ± 89 | 3 | 660 ± 139 | 3 | 942 ± 526 | n.s |
Cer 24:1 | |||||||
Main bronchus | 3 | 334 ± 274 | 3 | 533 ± 136 | 3 | 479 ± 202 | n.s |
Bronchus | 3 | 222 ± 101 | 3 | 445 ± 170 | 3 | 271 ± 178 | n.s |
Periphery | 3 | 447 ± 194 | 3 | 546 ± 38 | 3 | 691 ± 238 | n.s |
Cer total | |||||||
Main bronchus | 3 | 1834 ± 1211 | 3 | 2387 ± 1024 | 3 | 1917 ± 339 | n.s |
Bronchus | 3 | 1278 ± 604 | 3 | 3076 ± 1216 | 3 | 1322 ± 426 | n.s |
Periphery | 3 | 2052 ± 342 | 3 | 2577 ± 623 | 3 | 4398 ± 2210 | n.s |
