Figure 3
From: Carotid smooth muscle contractility changes after severe burn
(a) Masson’s trichrome stain shows significantly increased collagen expression within the tunica media smooth muscle of the carotid artery at 1 day and 3 days after burn compared to sham-treated animals. Top row: Masson’s trichrome stain (MT); collagen fibers stain blue, muscle fibers stain red. Middle row: Color thresholding ImageJ method shows total collagen in black. Bottom row: Adventitia tissue digitally removed, leaving only collagen stained within the tunica media. (b) Quantification of the percent area occupied by collagen within tunic media; data is presented as mean % Area Collagen ± SD. Scale bar = 50 µm. *p < 0.05, ***p < 0.001. One-way ANOVA with Bonferroni t-tests was applied.
