Figure 3

Flow cytometric analysis of CD14 selected monocytes used for dendritic cells differentiation. Flow cytometry dot plot analysis (A) Exclusion of doublets, gating on FSC-Area vs FSC-High; (B) sequential gate on cells expressing CD45; (C) and a final gate displaying 99% purity, almost all cells are identified as monocytes by the co-expression of CD14+ and CD33+.