Figure 1

Overview of the different steps involved in the PhageTermVirome workflow. Virome sequencing reads are simultaneously mapped to numerous contigs assembled from the virome dataset. After alignments for each contig, PTV performs peak detection and statistical analyses to determine the position of significant termini. On all contigs for which termini were detected, a coverage pattern analysis is achieved to predict the corresponding genome packaging mechanism. ^aThe sequencing method and library preparation require the preservation of genome ends (ex: all methods involving random DNA fragmentation prior to library preparation and long-read sequencing of intact DNA). ^bPhageTermVirome can process massive amount of contigs as input (multifasta), but still allow users to analyze a single phage contig (fasta).