Figure 2 | Scientific Reports

Figure 2

From: Porphyromonas gingivalis induces entero-hepatic metabolic derangements with alteration of gut microbiota in a type 2 diabetes mouse model

Figure 2

Parallel reaction monitoring analysis of the distinct peptides derived from Porphyromonas gingivalis (Pg). (a) Chromatogram of the fragment ions for the Pg-unique peptide, DVTVEGSNEFAPVQNLTGSAVGQK and its five different amino-acid fragments in Pg- or control-treated mouse feces, and that of the synthetic peptide used as the internal standard. (b) Detection of this peptide in Pg-treated, but not CMC control-treated, mice, using a synthetic peptide as internal standard. Sample preparation was performed in triplicate from the pooled feces; peptides were quantified based on the median peak area ratio of each fragment ion. (c) Phylum-, (d) family-, and (e) genus-level distributions of the fecal microbiome. Taxonomic assignments of the peptides identified via metaproteome analysis of fecal samples were performed using Unipept. The distributions were profiled based on the number of taxa-specific peptides. ‘Control 1 day’: CMC control-treated, 1 d after the first injection; ‘Control 30 days’: CMC control-treated, 30 d after the tenth injection; ‘Pg 1 day’: Pg-treated, 1 d after the first injection: ‘Pg 30 days’: Pg-treated, 30 d after the tenth injection.

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