Figure 3
From: Development of CIDEA reporter mouse model and its application for screening thermogenic drugs
Specificity of fluorescence reporter expression in brown and beige adipose tissue of the CIDEA reporter mice. (a) Immunofluorescence staining of CIDEA and tdTomato in adipose tissue frozen sections of homozygous (HOMO) CIDEA reporter mice. Nuclei were counterstained with DAPI (Blue). Bar = 20 μm. (b) Comparison of adipose tissue frozen section (BAT) or whole mount (iWAT and gWAT) of HOMO CIDEA reporter (TG) and wild type (WT) mice. Mice were maintained in cold (4 °C for a week) or room temperature (22 °C) condition. Nuclei and lipid droplets were counterstained with DAPI and HCS LipidTox deep red. Bar = 50 μm. (c) Ex vivo fluorescence imaging of tissues freshly isolated from WT, Heterozygous (HET), and HOMO CIDEA reporter mice. Quantification of fluorescence signal was normalized to each tissue weight. Data were analyzed by an unpaired, two-tailed t test (mean ± SEM; n = 3 *P < 0.05).
