Figure 6
From: Development of CIDEA reporter mouse model and its application for screening thermogenic drugs
Analyses of browning effects of PMSFs by using in vitro CIDEA reporter primary culture system. Browning effects of polymethoxyselenoflavones (PMSFs) were tested utilizing in vitro CIDEA reporter primary culture system. Differentiated adipocytes from BAT were treated with the indicated compound (1 μM) for 24 h. (a) IUPAC name and structure of PMSFs. (b) Fluorescence microscope images and quantification of fluorescence intensity. (c) In vitro bioluminescence imaging. Cells were treated with growth medium containing d-luciferin (150 μg/ml). (d) Luciferase assay of cell lysate. (e) Immunoblot analysis and quantification. Statistical analyses were assessed with an unpaired, two-tailed t test in (b), (d–e) (mean ± SEM; n = 3–4, *P < 0.05, **P < 0.01, and ***P < 0.001).
