Figure 2
From: DAPL1 is a novel regulator of testosterone production in Leydig cells of mouse testis
Experimental design and confirmation of Dapl1 deletion. (A) Schematic representation for the CRISPER/Cas9-mediated gene deletion used for the generation of Dapl1-KO mice. F and R corresponds to Dapl1 Forward and Reverse primers respectively, for the coding region of interest employed for assessing genotyping experiments. (B) The diagnostic patterns of three Dapl1 genotypes are shown. Full-length agarose gel for (B) is presented in Supplementary Fig. S6. (C) Experimental design of the study. Ex exon, KO homo-knockout, Het heterozygote, WT wild-type, PND postnatal day. Some illustrations used in this figure were originated from leased Motifolio (Scientific Illustration Toolkits for Presentations and Publications) materials.
