Figure 3
From: DAPL1 is a novel regulator of testosterone production in Leydig cells of mouse testis
Effects of Dapl1 deletion on sex steroid hormone synthesis in mouse testes at PND49. (A) Effect of Dapl1 ablation on the testicular steroidogenesis gene-expression of mRNA coding for StAR, CYP11A1, CYP17A1, 3β-HSD, and 17β-HSD in male mice at PND49. Each bar represents the mean ± S.E.M. of 9–12 mice. (B) Effect of Dapl1 ablation on the testicular expression of StAR protein in the mice at PND49. The representative electrophoretic image of each group is shown in the upper panels. The relative level of StAR expression was calculated by normalization to β-actin. Each bar represents the mean ± S.E.M. of 5–6 mice. (C) Effect of Dapl1 ablation on circulating levels of testosterone in male mice at PND49. Each bar represents the mean ± S.E.M. of 6–7 mice. (D) Effect of Dapl1 ablation on the testicular steroidogenesis gene-expression of mRNA coding for SRD5A1 and SRD5A2 in male mice at PND49. Each bar represents the mean ± S.E.M. of 9–11 mice. Significantly different from the wild-type control: *p < 0.05. DAPL1 death-associated protein-like 1, StAR steroidogenic acute regulatory protein, CYP cytochrome P450, 3β-HSD 3β-hydroxysteroid dehydrogenase, 17β-HSD 17β-hydroxysteroid dehydrogenase, SRD5A1 steroid 5α-reductase 1, SRD5A2 steroid 5α-reductase 2, PND49 postnatal day 49. Full-length blots for (B) are presented in Supplementary Figs. S7 and S8.
