Figure 6

Effects of Dapl1 deletion on the PKA system and CREB/CREM pathway in mouse testes at PND49. (A) Effect of Dapl1 ablation on the testicular CREB/CREM pathway gene-expression of mRNA coding for CREB1, CRTC1, CREM, and ACT in mice at PND49. Each bar represents the mean ± S.E.M. of 9–12 mice. (B) Effect of Dapl1 ablation on the testicular PKA system gene-expression of mRNA coding for PRKACα in mice at PND49. Each bar represents the mean ± S.E.M. of 9–12 mice. (C) Effect of Dapl1 ablation on gene-expression of mRNA coding for AKAP1 in mice testes at PND49. Each bar represents the mean ± S.E.M. of 11–12 mice. (D) Effect of Dapl1 ablation on the testicular MAPK/ERK pathway gene-expression of mRNA coding for MAPK1 and MAPK3 in mice at PND49. Each bar represents the mean ± S.E.M. of 11–12 mice. Significantly different from the wild-type control: *p < 0.05, **p < 0.01. DAPL1 death-associated protein-like 1, CREB cyclic AMP-response element-binding protein; CREM CRE modulator protein; CRTC1 CREB regulated transcription coactivator 1; ACT activator of CREM in testis; PRKACα protein kinase, cAMP-dependent, catalytic, α; AKAP1 A-kinase anchor protein 1; MAPK mitogen-activated protein kinase; PND49 postnatal day 49.