Figure 7

Effects of Dapl1 transfection on the PKA-CREB/CREM-StAR pathway in I-10 cells. (;) The effect of Dapl1 transfection and PKA modulating agents on the PKA system and CREB/CREM pathway gene-expression of mRNA coding for StAR, PRKACα, CREB1, and CREM in I-10 cells. We used an empty plasmid to transfect the "pcDNA3.1-Dapl1 (−)" group of cells, and used the pcDNA3.1-mouse Dapl1 expression plasmid to transfect the "pcDNA3.1-Dapl1 (+)" group of cells. After transfection, the samples were incubated for 48 h, and the related drugs dissolved in DMSO were added. Among them, only the “pcDNA3.1-Dapl1 (−)” group sample with DMSO solvent was added as a control group. Each bar represents the mean ± S.E.M. of 3–4 samples. (B) Effect of Dapl1 transfection on the MAPK/ERK pathway gene-expression of mRNA coding for MAPK1 and MAPK3 in I-10 cells. Details of transfection are described in the legend to Fig. 5 and the Material and Methods. Each bar represents the mean ± S.E.M. of 5–6 samples. (C) Effect of Dapl1 transfection on the CREB/CREM pathway gene-expression of mRNA coding for CRTC1 in I-10 cells. Each bar represents the mean ± S.E.M. of 5–6 samples. (D) Effect of Dapl1 transfection on the gene-expression of mRNA coding for AKAP1 in I-10 cells. Each bar represents the mean ± S.E.M. of 5–6 samples. Significantly different from the control I-10 cells: *p < 0.05, **p < 0.01, ***p < 0.001. DAPL1 death-associated protein-like 1, StAR steroidogenic acute regulatory protein, PKA protein kinase A, CREB cyclic AMP-response element-binding protein, CREM CRE modulator protein, CRTC1 CREB-regulated transcription coactivator 1, AKAP1 A-kinase anchor protein 1, MAPK mitogen-activated protein kinase.