Figure 2 | Scientific Reports

Figure 2

From: Prolyl isomerase Pin1 plays an essential role in SARS-CoV-2 proliferation, indicating its possibility as a novel therapeutic target

Figure 2

Pin1 inhibitor H-77 inhibits SARS-CoV-2 proliferation in a concentration-dependent manner. VeroE6/TMPRSS2 cells were pretreated with the indicated concentrations of H-77 for 2 h, and were subjected to SARS-CoV-2 infection (MOI of 0.01) followed by incubation for 24 h in the presence of the same concentrations of H-77. (A) Western blotting of cell lysates (Duplicate in the figure) with SARS-CoV-2 N, Pin1, and actin antibodies. Full-length blots are presented in Supplementary Fig. 4. (B) vRNA copies contained in 5 µL of the supernatant were quantified by RT-qPCR using authorized SARS-CoV-2 N primers (n = 3). (C) Fusion index calculated by counting numbers of cells and nuclei of infected cells after Giemsa straining. Approximately 100 nuclei and cell number per field were counted for each of five fields. Error bars indicate standard deviation. *P < 0.05, **P < 0.01, Mann–Whitney U test, compared with 0 µM (DMSO). (D) Representative images of SARS-CoV-2-infected VeroE6/TMPRSS2 cells at 24 h post-infection with the indicated concentrations of H-77.

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