Table 1 Purification scheme of the CapA.
Purification steps | Total protein (mg) | Total activity (U) | Specific activity (U mg−1) | Enrichment (fold) | Yield (%) |
---|---|---|---|---|---|
Crude extract | 124.5 | 26,057.9 | 209.3 | 1 | 100 |
Ammonium sulfate precipitation and dialysis | 44.6 | 17,282.5 | 387.5 | 1.9 | 66 |
Q-Sepharose | 2.8 | 1388 | 495.7 | 2.4 | 5 |