Figure 7
From: Role of pyruvate in maintaining cell viability and energy production under high-glucose conditions
Benfotiamine prevents IMS32 cell death and ATP depletion without accelerating purine metabolism under high-glucose pyruvate-starved conditions. Cell viability (A) at 24 h and intracellular ATP content (B) at 3 h in the Glc 5 mM/Pyr (+)] (blue), [Glc 5 mM/Pyr (−)] (yellow), [Glc 15 mM/Pyr (+)] (brown), [Glc 15 mM/Pyr (−)] (green) and [Glc 15 mM/Pyr (−)/Benfotiamine (+)] (red), [Glc 15 mM/Pyr (−)/6-mercaptopurine (6-MP) (+)] (purple), and [Glc 15 mM/Pyr (−)/Benfotiamine (+)/6-MP (+)] (black) group were determined by MTS assay (A) and CellTiter Glo 2.0 assay (B), respectively. Values represent mean + SD from nine (A) and 10 (B) experiments (individual values are depicted as circles, triangles, pluses, crosses and asterisks). * P < 0.05, ** P < 0.01. 6-MP, 6-mercaptopurine.
