Figure 2

The dispensable role of Gsdmd in the development of autoimmune arthritis. (A) The arthritis scores of WT and Gsdmd^−/− SKG mice (n = 8 each) after the injection of mannan (20 mg). (B) The IL-1β concentration of synovial tissues from inflamed joints. (C,D) Flow cytometry of IL-17A⁺, GM-CSF⁺, IFN-γ⁺ and Foxp3⁺ cells in CD4⁺ T cells in draining LNs and inflamed joints of WT and Gsdmd^−/− SKG mice at 10 weeks after mannan injection (n = 8 each). (E) Experimental design of adoptive transfer of CD4⁺ T cells from WT or Gsdmd^−/− SKG mice into Rag2^−/− or Gsdmd^−/− Rag2^−/− mice. (F) The arthritis scores of the four groups (a-d) shown in (E) (n = 9–10 each). (G,H) Flow cytometry of IL-17A⁺, GM-CSF⁺, IFN-γ⁺ and Foxp3⁺ cells in CD4⁺ T cells in draining LNs and inflamed joints of the four groups of mice at 12 weeks after cell transfer (n = 8–10 each). The vertical bars denote the SD in panels (B,D) and SEM in (A,F). The horizontal line in panel (H) denotes the mean. *P < 0.05. Data are pooled from two independent experiments in panels (A,B,D,F,H).