Figure 5

Macrophage autophagy invalidation affects the splenic immune response. (A) Left, spleens of WT or ATG5^Mye−/− mice 10 months after DEN treatment; Right, spleen weight and spleen/body weight ratio of WT or ATG5^Mye−/− mice 10 months after DEN or saline solution administration. (B) Number of splenic macrophages and MFI of PD-L1 in splenic macrophages in WT or ATG5^Mye−/− mice 10 months after DEN or saline solution administration. (C) Number of splenic CD3⁺, CD4⁺ and CD8⁺ T cells in WT or ATG5^Mye−/− mice 10 months after DEN or saline solution administration. (D) Number of splenic Treg cells and CD8 + cells/Treg cells ratio in WT or ATG5^Mye−/− mice 10 months after DEN or saline solution administration. Data are shown as mean ± SEM. *p < 0.05 for WT Control vs WT DEN, # for ATG5^Mye−/− Control vs ATG5^Mye−/− DEN and ^&p < 0.05 for WT vs ATG5^Mye−/−. n = 5–8 for WT mice Control; n = 4–5 for ATG5^Mye−/− mice Control; n = 8–12 for WT mice DEN; n = 4–7 for ATG5^Mye−/− mice DEN.