Figure 7
From: Macrophage autophagy protects against hepatocellular carcinogenesis in mice
Tumor cells inhibit macrophage autophagy. (A) Representative western blot analysis of LC3 and β-actin in peritoneal macrophages exposed to the conditioned medium of Hepa1-6 cells (CMH) or to control medium (CM) in the presence or absence of 10 µM chloroquine (CQ). (B) Representative western blot analysis of LC3 and β-actin in peritoneal macrophages exposed to the conditioned medium of AML-12 cells (CMA) or to control medium (CM) in the presence or absence of 10 µM chloroquine (CQ). (C) Representative western blot analysis of P-mTOR, mTOR, P-S6rp and S6rp in peritoneal macrophages exposed to the conditioned medium of Hepa1-6 cells (CMH) or to control medium (CM) in the presence or absence of 10 µM chloroquine (CQ). (D) Representative western blot analysis of P-p38 and p38 in peritoneal macrophages exposed to the conditioned medium of Hepa1-6 cells (CMH) or to control medium (CM) in the presence or absence of 10 µM chloroquine (CQ). (E) Representative western blot analysis of LC3 and β-actin in peritoneal macrophages exposed to the conditioned medium of Hepa1-6 cells (CMH) or to control medium (CM) in the presence or absence of 10 µM chloroquine (CQ) and of 10 µM SB202199.
