Figure 1
White spots and ‘rosettes’ in early postnatal C57BL/6J Nr2e3rd7/rd7 retinas. Fundus examination (upper panels) and representative OCT imaging (lower panels) on C57BL/6J Nr2e3rd7/rd7 eyes at postnatal day (P) 12 (A), P13 (B), P15 (C), P18 (D), P21 (E) and P28 (F–H). At P12 and P13 white spots are indicated by white arrows because retinal imaging is hampered by the developmental opacity of the lens (A,B). At P28, the region of the OCT section is indicated by a red line (G,H), and colocalization of white spots and ‘rosettes’ indicated by color-coded arrows (H). Scale bars: 50 µM. (I) Quantification of ‘rosettes’ in histological sections of C57BL/6J Nr2e3rd7/rd7 retinas during postnatal development. Three retinas from three different mice were analyzed. Per retina, the number of ‘rosettes’ was counted on five hematoxylin–eosin stained sections along a dorso-ventral axis containing the optic nerve head. Statistical analysis was performed by ordinary one-way ANOVA with Tukey’s multiple comparisons test. *p < 0.05 ***p < 0.001; ****p < 0.0001. (J) Qualitative spatio-temporal distribution of rosettes analyzed in graph I along a dorso-ventral axis relative to the optic nerve head (0).
