Figure 3

In vivo imaging of Iba1 expressing cells in Cx3cr1^gfp/+ and Nr2e3^rd7/rd7Cx3cr1^gfp/+ retinas. Fundus autofluorescence imaging of Nr2e3^rd7/rd7 Cx3cr1^gfp/+ retinas identify GFP-positive cells as a white signal (A). Hyperautofluorescent signals colocalize with ‘rosettes’ on OCT B-scans (B; scan indicated by green line and ‘rosette’ delimitated by yellow bars) and magnification shows accumulation of individual hyperautofluorescent cells (C). Multiple hyperautofluorescent signals are detected at P28 by fundus autofluorescence imaging of Nr2e3^rd7/rd7 Cx3cr1^gfp/+ retinas (D; white arrows), but not Cx3cr1^gfp/+ retinas (E). Longitudinal in vivo analysis of hyperautofluorescent signals in Nr2e3^rd7/rd7 Cx3cr1^gfp/+ retinas at P21 (F), P28 (G) and P60 (H). Unique signals are indicated by white arrows, repeatedly detected signals by color-coded arrows.