Figure 4
In vivo and in vitro analysis of outer nuclear layer thickness in adult C57BL/6J Nr2e3rd7/rd7 retinas. Fundus examination (upper panels) and OCT imaging (lower panels) on C57BL/6J Nr2e3rd7/rd7 retinas at 7 (A), 9 (B), 12 (C) and 15 (D) months (m), and on C57BL/6J retinas at 6 (E) and 18 (F) months. The red line on fundus images indicates the OCT scan. (G) Quantification of the outer nuclear layer (ONL) thickness by OCT. Average of 4 eyes from 4 different mice ± SD per time point with 7–13 ONL thickness measurements along a 2.2 cm dorso-ventral axis centered on the optic nerve head and avoiding rosette-containing regions (n = 29–87). Statistical analysis was performed by ordinary one-way ANOVA with Sidak’s multiple comparisons test. **p < 0.01; ****p < 0.0001. By performing an unpaired two-tailed t test between wild-type 6 m and 18 m a significant P value of 0.0004 is observed. Fundus photography on C57BL/6J Nr2e3rd7/rd7 retinas at 24 (H), 28 (I) and 31 (J) months (m). (K) Quantification of outer nuclear layer (ONL) thickness assessed by the number of rows of nuclei on hematoxylin–eosin-stained sections of retinas from 3 eyes ± SD of 30-month-old C57BL/6J (BL6) and 31-month-old C57BL/6J Nr2e3rd7/rd7 (rd7) mice. Five ONL thickness measurements along a dorso-ventral axis centered on the optic nerve head were done. Statistical analysis was performed by unpaired t-test. ****p < 0.0001.
