Figure 6
Cell death mechanisms in C57BL/6J Nr2e3rd7/rd7 retinas. Immunohistochemical analysis of cell death markers on 5 μM-paraffin sections of 1-year-old (1y) C57BL/6J Nr2e3rd7/rd7 (rd7) (A,C,E,G,I,K,M) and wild-type C57BL/6J (BL6) (B,D,F,H,J,L,N) retinas. Sections were probed with antibodies raised against Bax (A,B), cleaved Caspase-3 (C,D), cleaved PARP-1 (E,F), MLKL (G,H), PAR (I,J), Calpain-2 (K,L) and Survivin (M,N). Bax, cleaved Caspase-3, MLKL and Survivin were detected with a secondary antibody conjugated to Alexa Fluor 594 (red), cleaved PARP-1 and Calpain-2 with a secondary antibody conjugated to Cy5 (red) and PAR with a secondary antibody conjugated to FITC (green). Nuclei were stained with DAPI (blue), namely the outer nuclear layer (ONL) and the inner nuclear layer (INL). Scale bar: 50 µm. (O) Qualitative Western blot analysis on six pooled retinas of 21-day (P21) and 1-year-old (1y) C57BL/6J (BL6) and C57BL/6J Nr2e3rd7/rd7 (rd7) mice. Expression of the 16-kDa survivin and 49-kDa α-tubulin proteins were assessed.
