Figure 2 | Scientific Reports

Figure 2

From: Derivation and characterisation of endothelial cells from patients with chronic thromboembolic pulmonary hypertension

Figure 2

CTEPH-EC characterization. (a) Images showing expansion of CTEPH-EC isolated from PEA specimen, (i) minced PEA material, (ii) endothelial colony at day 10, (iii) day 14 and (iv) confluent CTEPH-EC. (b) Representative flow cytometry histograms of HPAE, CTEPH-EC and smooth muscle cells (SMC) labelled with antibodies against endothelial surface makers (CD31, UEA-1, vWF, VE-CAD, eNOS) and muscular markers (α-SMA and calponin) (n = 10). (c) Representative images of HPAE, CTEPH-EC, and SMC were immune-labelled with antibodies against endothelial makers (CD31, CAV1, vWF, VE-CAD, eNOS) and muscular markers (α-SMA and calponin) (n = 10). Nuclei were counterstained using DAPI (blue) × 40 magnification.

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