Figure 3
Expression of cell-specific markers in CTEPH-EC. (a) CTEPH-EC presented endothelial markers at the mRNA level and were negative for muscular markers (myocardin). Values expressed as mean ± SD, n = 5 HPAE, n = 11 CTEPH-EC and n = 8 SMC independent experiments performed in triplicate, p < 0.05*, p < 0.01**, p < 0.0001****, Mann–Whitney U test comparing two groups and Kruskal–Wallis test followed by Dunn’s multiple comparisons test for three groups. (b) CD31, eNOS, vWF and Cav-1 protein levels in CTEPH-EC compared to HPAE. Values expressed as mean ± SD, n = 3 HPAE, n = 7 CTEPH-EC independent experiments, p < 0.05*, Mann–Whitney U test, (full-length gels are presented in supplementary material Fig. 7). The intensity of the individual bands was quantified using freely available Image Lab software (Biorad laboratories), version 6.1.0 build7, http://www.biorad.com. (c) CTEPH-EC and HPAE expression levels of HOXD3, -8 and -9 compared to HMVEC-L. Values expressed as mean ± SD, n = 5 HPAE, n = 9 CTEPH-EC and n = 5 HMVEC-L independent experiments performed in triplicate, p < 0.05*, p < 0.01**, Kruskal–Wallis test followed by Dunn’s multiple comparisons test. Statistical analysis was performed with GraphPad Prism 7 software, version 7.0e, serial number:GP7-0633739-R###-#####, https://www.graphpad.com.
