Figure 4
Proliferation capacities of CTEPH-EC. (a) Proliferative capacity of CTEPH-EC as population doubling time. n = 3 HPAE, CTEPH-EC, n = 5 independent experiments performed in triplicate (b) Viability of CTEPH-EC compared to HPAE evaluated by MTT assay. Values expressed as mean ± SD, n = 5 HPAE, n = 8 CTEPH-EC independent experiments performed in triplicate, p < 0.05*, p < 0.01**, Mann–Whitney U test. (c) Quantification and stain of proliferative marker Ki-67 in CTEPH-EC and HPAE at different passages. Values expressed as mean ± SD, n = 5 HPAE, n = 8 CTEPH-EC; independent experiments p < 0.05*, p < 0.01**, Mann–Whitney U test, (d) Clonogenic potential of CTEPH-EC expressed in percentage. Values expressed as mean ± SD, n = 6 HPAE, n = 6 CTEPH-EC; independent experiments, p < 0.05*, Mann–Whitney U test. (E) Cellular adhesion of CTEPH-EC compared to HPAE. n = 3 HPAE, n = 6 CTEPH-EC. Statistical analysis was performed with GraphPad Prism 7 software, version 7.0e, serial number:GP7-0633739-R###-#####, https://www.graphpad.com.
