Figure 5 | Scientific Reports

Figure 5

From: Derivation and characterisation of endothelial cells from patients with chronic thromboembolic pulmonary hypertension

Figure 5

Apoptosis in CTEPH-EC. (a) mRNA expression profile of apoptotic markers caspase-3, -8 and -9 in CTEPH-EC compared to HPAE. Values expressed as mean ± SD, n = 5 HPAE, n = 11 CTEPH-EC independent experiments performed in triplicate, p < 0.05*, Mann–Whitney U test. (b) Cellular perimeter, area, and diameter of CTEPH-EC and HPAE measured at different passages. Values expressed as mean ± SD, n = 8 HPAE, n = 8 CTEPH-EC independent experiments performed in triplicate. p < 0.05*, p < 0.0001****, two-way ANOVA followed by Tukey´s multiple comparisons test. (c) Representative images of CTEPH-EC and HPAE cells at different cell passages. Cellular circumference, area and diameter were measured in triplicate fields of 5–10 cells/picture (20 × magnification) using freely available imaging processing ImageJ software, version:2.1.0/1.53c, http://imagej.net/contributors. Statistical analysis was performed with GraphPad Prism 7 software, version 7.0e, serial number:GP7-0633739-R###-#####, https://www.graphpad.com.

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