Figure 8
Oxidative stress in CTEPH-EC. (a) ROS levels in CTEPH-EC and HPAE were visualized by fluorogenic dye MitoSOX (red) n = 5 HPAE, n = 7 CTEPH-EC independent experiments, p < 0.01**, Mann–Whitney U test, values expressed as mean ± SD and CellROX (green) n = 6 HPAE, n = 6 CTEPH-EC, p > 0.05. (b) Oxidation status of proteins in CTEPH-EC, HPAE, patient serum and serum from healthy volunteers. n = 3 HPAE, n = 7 CTEPH-EC; n = 3 HPAE, n = 6 HV, p < 0.05*, Mann–Whitney U test, values expressed as mean ± SD. The intensity of the bands was quantified using Image Lab software (Biorad laboratories), version 6.1.0 build7, http://www.biorad.com. (c) mRNA expression of SOD1 and SOD2 in CTEPH-EC and HPAE. Values expressed as mean ± SD, n = 6 HPAE, n = 10 CTEPH-EC independent experiments performed in triplicate, p < 0.05*, Mann–Whitney U test. (d) Staining of DNA damage induced by oxidative stress in PEA specimen using 8-hydroxy-2′-deoxyguanosine (8-OHdG). Statistical analysis was performed with GraphPad Prism 7 software, version 7.0e, serial number:GP7-0633739-R###-#####, https://www.graphpad.com.
